Difference between revisions of "Part:BBa K3962354"
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<partinfo>BBa_K3962354 short</partinfo> | <partinfo>BBa_K3962354 short</partinfo> | ||
| − | This construct is composed of L-arabinose | + | This construct is composed of L-arabinose mediated inducible promoter pBAD and antitoxin MazE of MazE/MazF toxin-antitoxin system. MazE/MazF is a type II toxin-antitoxin system originating from E. coli. This construct can be used to characterize the antagonistic effect of MazE when combining with MazE expression constructs. It can also be used as a biocontainment component. Because when bacteria escape to the natural environment without the inducers the antitoxin gene is repressed. |
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Latest revision as of 08:09, 16 October 2021
Inducible expression of antitoxin MazE by pBAD
This construct is composed of L-arabinose mediated inducible promoter pBAD and antitoxin MazE of MazE/MazF toxin-antitoxin system. MazE/MazF is a type II toxin-antitoxin system originating from E. coli. This construct can be used to characterize the antagonistic effect of MazE when combining with MazE expression constructs. It can also be used as a biocontainment component. Because when bacteria escape to the natural environment without the inducers the antitoxin gene is repressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 1283 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961