Difference between revisions of "Part:BBa K3930006"
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<p>The Z3eV assembly  is composed by the promoter TEF1, the gene <i>Z3eV</i> coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al. 2020). </p>
 
<p>The Z3eV assembly  is composed by the promoter TEF1, the gene <i>Z3eV</i> coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al. 2020). </p>
 
<p>This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into <i>S.cerevisiae</i>.  
 
<p>This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into <i>S.cerevisiae</i>.  
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<h2>References</h2>
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    <li>Liu J, Wang T, Jiang Y, Liu Z, Tian P, Wang F, Deng L. 2020. Harnessing β-estradiol inducible expression system to overproduce nervonic acid in Saccharomyces cerevisiae. Process Biochemistry. 92:37–42. doi:10.1016/j.procbio.2020.02.032.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:20, 13 October 2021


Inducer Z3EV of the estradiol promoter Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
    Illegal AgeI site found at 1527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1849
    Illegal BsaI.rc site found at 987


Introduction

The Z3eV assembly is composed by the promoter TEF1, the gene Z3eV coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al. 2020).

This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into S.cerevisiae.

References

  1. Liu J, Wang T, Jiang Y, Liu Z, Tian P, Wang F, Deng L. 2020. Harnessing β-estradiol inducible expression system to overproduce nervonic acid in Saccharomyces cerevisiae. Process Biochemistry. 92:37–42. doi:10.1016/j.procbio.2020.02.032.

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