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Revision as of 21:44, 12 October 2021


Galactose inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

The promoter Gal1 is an inducible promoter upon addition of galactose into the media of culture. This sequence comes from the Sacharomyces cerevisiae genome. This part is related to the extensively used and characterized part BBa_J63006. Nonetheless, our promoter lack the kozak sequence.

Results

Production of α-ionone (BBa_K3930003)

The part (BBa_K3930003) was linearized and transformed into the S.cerevisiae LycoYeast strain. The production of α-ionone was induced by plating the transformant onto a YPGal media (20mg.ml-1 of galactose). Figure 1 shows the mass spectra between an induced and a non-induced strain.



Figure 1: Production of α-ionone upon galactose activation

α-ionone is produced in vivo by our strain when it is induced by galactose. On the right are presented the mass spectra that correspond between the standard and the observed peak


This promoter gal without the kozak sequence work under those lab conditions.