Difference between revisions of "Part:BBa K3853008"
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− | Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. This is the version with SpyTag. In order to be able to construct our multi-enzyme complex, we introduced the SpyTag/SpyCatcher system. We added SpyTag to the N-terminus of each protein, so it can be combined with dCas9-SpyCatcher through covalent isopeptide action, thereby being immobilized On dsDNA. His-tag was added to purify the protein. We use <partinfo>BBa_K3853051</partinfo> to construct the expression system and to express and to purify the protein. | + | Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. This is the version with SpyTag. In order to be able to construct our multi-enzyme complex, we introduced the SpyTag/SpyCatcher system. We added SpyTag to the N-terminus of each protein throught 10 × ELP, so it can be combined with dCas9-SpyCatcher through covalent isopeptide action, thereby being immobilized On dsDNA. His-tag was added to purify the protein. We use <partinfo>BBa_K3853051</partinfo> to construct the expression system and to express and to purify the protein. |
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Revision as of 20:00, 12 October 2021
his-tag-SpyTag-MnP
Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. This is the version with SpyTag. In order to be able to construct our multi-enzyme complex, we introduced the SpyTag/SpyCatcher system. We added SpyTag to the N-terminus of each protein throught 10 × ELP, so it can be combined with dCas9-SpyCatcher through covalent isopeptide action, thereby being immobilized On dsDNA. His-tag was added to purify the protein. We use BBa_K3853051 to construct the expression system and to express and to purify the protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]