Difference between revisions of "Part:BBa K3887000"
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=Description= | =Description= | ||
Fre is a flavin reductase that regenerates FADH2 and highly expressed as a soluble form in E. coli, L3 is a rigid linker commonly used for fusion enzymes. | Fre is a flavin reductase that regenerates FADH2 and highly expressed as a soluble form in E. coli, L3 is a rigid linker commonly used for fusion enzymes. | ||
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In our experiment, Fre-L3 is used as a N-terminal tag and cofactor supply with three halogenases (SttH, PyrH, RebH) as strategy to enhance the solubility of halogenases in vivo. Thus, the fusion enzymes could compatible with whole-cell reactions or metabolic engineering. It is necessary to increase their activity as well as the yield of target molecules. | In our experiment, Fre-L3 is used as a N-terminal tag and cofactor supply with three halogenases (SttH, PyrH, RebH) as strategy to enhance the solubility of halogenases in vivo. Thus, the fusion enzymes could compatible with whole-cell reactions or metabolic engineering. It is necessary to increase their activity as well as the yield of target molecules. | ||
Latest revision as of 06:01, 12 October 2021
Fre-L3
A soluble partner and cofactor supply for fusion enzyme.
Description
Fre is a flavin reductase that regenerates FADH2 and highly expressed as a soluble form in E. coli, L3 is a rigid linker commonly used for fusion enzymes.
In our experiment, Fre-L3 is used as a N-terminal tag and cofactor supply with three halogenases (SttH, PyrH, RebH) as strategy to enhance the solubility of halogenases in vivo. Thus, the fusion enzymes could compatible with whole-cell reactions or metabolic engineering. It is necessary to increase their activity as well as the yield of target molecules.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]