Difference between revisions of "Part:BBa K3982007:Design"
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===Source===
 
===Source===
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NCBI Reference Sequence: NC_000962.3
 
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Reference: L. B. Harrington et al. Science 10.1126/science.aav4294 (2018).
 
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===References===
 
===References===
 
1) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z
 
1) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z
 
2) NCBI Reference Sequence for Mycobacterium tuberculosis H37Rv: NC_000962.3
 
2) NCBI Reference Sequence for Mycobacterium tuberculosis H37Rv: NC_000962.3

Revision as of 12:11, 11 October 2021


CODE M sgRNA for targeting wildtype rpoB gene in Mycobacterium tuberculosis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The scaffold region has been designed as referred from Ref. 1. It consists of the tracrRNA and regions of crRNA. The guiding region is the 20 bp spacer sequence which is complementary to the rpoB gene in Mtb and referred from Ref 2.

The 20 bp sequence is as follows: cgccgactgtcggcgctggg

Source

References

1) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z 2) NCBI Reference Sequence for Mycobacterium tuberculosis H37Rv: NC_000962.3