Difference between revisions of "Part:BBa K3962339:Design"

 
 
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===Design Notes===
 
===Design Notes===
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As it was used as reference of fluorescent protein expression, the subparts including the promoter, RBS, reporter and terminator were the most frequently used parts in the registry
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We designed this construct to calibrate the expression of pBAD to a standardized constitutive promoter. Codon optimization was performed for the expression of the gene in E. coli.
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Latest revision as of 14:14, 15 October 2021


J23100 promoter with mCherry for constant expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this construct to calibrate the expression of pBAD to a standardized constitutive promoter. Codon optimization was performed for the expression of the gene in E. coli.


Source

All parts were come from iGEM registry.

References