Difference between revisions of "Part:BBa K3895003"

Revision as of 15:27, 10 October 2021

Keratinase kerAvDZ50

KerAVDZ50 is an extracellular serine thiol alkaline protease from the Actinomadura viridilutea strain, which can be isolated from the Algerian fishing port [1]. As a thermo-alkaline keratinases, it can function at a pH range of 7–12 and a temperature range of 35–80 °C.

6x His-tags were added to both sides of the sequence for purification.

Modeling

Figure 1. Swiss Model showing serine residues of KerAVDZ50.

Figure 2. Swiss Model showing hydrophobisity of KerAVDZ50.

Figure 3. Ramachandran Plot of KerAVDZ50.Z50 Ramachandran Favoured is 95.14%

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 577
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 382
Illegal AgeI site found at 1120
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 322

Reference

Biochemical and molecular characterization of new keratinoytic protease from Actinomadura viridilutea DZ50. (n.d.). International Journal of Biological Macromolecules, 92, 299–315. https://doi.org/10.1016/j.ijbiomac.2016.07.009

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 <partinfo>BBa_K3895003 short</partinfo>
-KerAVDZ50 is an extracellular serine thiol alkaline protease from ''Actinomadura viridilutea'' strain, which can be isolated from Algerian fishing port [1]. As a thermo-alkaline keratinases, it can function at a pH range of 7–12 and a temperature range of 35–80 °C.
+KerAVDZ50 is an extracellular serine thiol alkaline protease from the ''Actinomadura viridilutea'' strain, which can be isolated from the Algerian fishing port [1]. As a thermo-alkaline keratinases, it can function at a pH range of 7–12 and a temperature range of 35–80 °C.
+x His-tags were added to both sides of the sequence for purification.
 ===Modeling===