Difference between revisions of "Part:BBa K3791014"

 
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Revision as of 12:01, 10 October 2021


gRNA Spectinomycin construct

This construct corresponds to the full DNA sequence that allows for the transcription of the guide RNA targeting spectinomycin resistance. For that purpose, in addition to the gRNA, it includes a promoter. Particularly, it is the T7 promoter, which used in pertinent competent cells is indirectly inducible by the addition of IPTG (as the T7 RNA-polymerase needs IPTG to be expressed) and therefore permits transcriptional modulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]