Difference between revisions of "Part:BBa K3791012:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | The repeat sequence must be placed before the spacer sequence, that is, in the 5’ extreme. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | The repeat and the T7 promoter sequences were extracted from the parts registry ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2927006 BBa_K2927006]) ([https://parts.igem.org/Part:BBa_K1614000 BBa_K1614000]), whereas the spacer one was obtained from being the complementary sequence of an erythromycin-resistant gene DNA fragment. (as explained in its own part page: [https://parts.igem.org/Part:BBa_K3791000 BBa_K3791002]). | |
===References=== | ===References=== | ||
| + | [1] New England Biolabs. (s/f). <b>How do I design a guide RNA for use with EnGen Lba Cas12a?</b> Neb.com. From: https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a | ||
Revision as of 11:57, 10 October 2021
gRNA Erythromycin construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The repeat sequence must be placed before the spacer sequence, that is, in the 5’ extreme.
Source
The repeat and the T7 promoter sequences were extracted from the parts registry (BBa_K2927006) (BBa_K1614000), whereas the spacer one was obtained from being the complementary sequence of an erythromycin-resistant gene DNA fragment. (as explained in its own part page: BBa_K3791002).
References
[1] New England Biolabs. (s/f). How do I design a guide RNA for use with EnGen Lba Cas12a? Neb.com. From: https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a