Difference between revisions of "Part:BBa K3885222"

 
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The short version of DNA sequence coding for CXCL9 mRNA can be expressed under the control of a strong promoter J23119.
 
The short version of DNA sequence coding for CXCL9 mRNA can be expressed under the control of a strong promoter J23119.
  
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===Usage and Biology===
 
===Usage and Biology===
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1.This plamsid can transfer CXCL9 mRNA. CXCL9 mRNA is a biomarker, based on the final intensity of the fluorescence, we can know if a person is sick.<br>
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2.It can be used in other experiments for disease markers.
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===Characterization===
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It can transfer CXCL9 mRNA to combine with the complex of igRNA and Cas9 protein. Then they can perform cutting function. And they can cut tetR of P70-σ28-P28-tetR to express fluorescence of P28-tetO-deGFP.
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===Design Page===
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CXCL9 mRNA is a biomarker of graft rejection. There is an application that some researchers applied and optimized the CRISPR–Cas13 specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) technology for diagnosis of biomarkers highly relevant for renal-trans-plant recipients[1]. So we use CRISPR-Cas9 to verify if this system can detect CXCL9 mRNA or other biomarkers.
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===References===
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Kaminski Michael M et al. A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection.[J]. Nature biomedical engineering, 2020, 4(6) : 601-609.
  
 
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Revision as of 10:55, 16 October 2021


J23119-CXCL9

The short version of DNA sequence coding for CXCL9 mRNA can be expressed under the control of a strong promoter J23119.

Usage and Biology

1.This plamsid can transfer CXCL9 mRNA. CXCL9 mRNA is a biomarker, based on the final intensity of the fluorescence, we can know if a person is sick.
2.It can be used in other experiments for disease markers.

Characterization

It can transfer CXCL9 mRNA to combine with the complex of igRNA and Cas9 protein. Then they can perform cutting function. And they can cut tetR of P70-σ28-P28-tetR to express fluorescence of P28-tetO-deGFP.

Design Page

CXCL9 mRNA is a biomarker of graft rejection. There is an application that some researchers applied and optimized the CRISPR–Cas13 specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) technology for diagnosis of biomarkers highly relevant for renal-trans-plant recipients[1]. So we use CRISPR-Cas9 to verify if this system can detect CXCL9 mRNA or other biomarkers.

References

Kaminski Michael M et al. A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection.[J]. Nature biomedical engineering, 2020, 4(6) : 601-609.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]