Difference between revisions of "Part:BBa K3885123"

 
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ClpXP consists of two distinct proteins, a AAA+ ATPase called ClpX（BBa_K1911003） and a peptidase called ClpP（BBa_K1911002）. We incorporate the ClpX and ClpP to be a one-part. In protein substrates, ClpX recognizes unstructured peptide sequences (called tags or degrons), like ssrA--a specific C-terminal degradation tag, proceeds to unfold stable tertiary structure in the protein, and then spools or translocates the unfolded polypeptide chain into a sequestered proteolytic compartment in ClpP for degradation into small peptide fragments (Fig. 1).  
 
ClpXP consists of two distinct proteins, a AAA+ ATPase called ClpX（BBa_K1911003） and a peptidase called ClpP（BBa_K1911002）. We incorporate the ClpX and ClpP to be a one-part. In protein substrates, ClpX recognizes unstructured peptide sequences (called tags or degrons), like ssrA--a specific C-terminal degradation tag, proceeds to unfold stable tertiary structure in the protein, and then spools or translocates the unfolded polypeptide chain into a sequestered proteolytic compartment in ClpP for degradation into small peptide fragments (Fig. 1).  
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Revision as of 07:54, 10 October 2021


ClpXP

ClpXP consists of two distinct proteins, a AAA+ ATPase called ClpX（BBa_K1911003） and a peptidase called ClpP（BBa_K1911002）. We incorporate the ClpX and ClpP to be a one-part. In protein substrates, ClpX recognizes unstructured peptide sequences (called tags or degrons), like ssrA--a specific C-terminal degradation tag, proceeds to unfold stable tertiary structure in the protein, and then spools or translocates the unfolded polypeptide chain into a sequestered proteolytic compartment in ClpP for degradation into small peptide fragments (Fig. 1).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
    Illegal BglII site found at 1628
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
    Illegal AgeI site found at 1110
    Illegal AgeI site found at 1848
  • 1000
    COMPATIBLE WITH RFC[1000]