Difference between revisions of "Part:BBa K3896006"

Latest revision as of 14:25, 10 October 2021

PmrCAB(HAPN)-LuxI-LuxR

We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of hAPN protein to accept the stimulation of S protein of 229E-CoV. After detecting the S protein of 229E-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.

The downstream genes will express AHL and LuxR, mediating the quorum sensing system. In the AHL mediated quorum sensing system composed of LuxI/LuxR, LuxI homologous gene in bacteria is responsible for the synthesis of QS signal molecule AHL, and LuxR homologue is activated as the receptor of this signal molecule to regulate the transcription of downstream genes.

figure(gene)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3503
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 637
Illegal BamHI site found at 3931
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2750
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3896006 short</partinfo>
-We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original pmrB with the core binding domain of hAPN protein to accept the stimulation of S protein of 229E-CoV. After detecting the S protein of 229E-CoV, the sensor kinase pmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.
+We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of hAPN protein to accept the stimulation of S protein of 229E-CoV. After detecting the S protein of 229E-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA. Then phosphorylated PmrA protein combined with promoter PmrC sequence to activate the expression of reporter gene.
 The downstream genes will express AHL and LuxR, mediating the quorum sensing system. In the AHL mediated quorum sensing system composed of LuxI/LuxR, LuxI homologous gene in bacteria is responsible for the synthesis of QS signal molecule AHL, and LuxR homologue is activated as the receptor of this signal molecule to regulate the transcription of downstream genes.