Difference between revisions of "Part:BBa K3755012:Design"
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===Source=== | ===Source=== | ||
| − | + | From literature of Eugene Kim's team from Washington University in St. Louis. | |
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===References=== | ===References=== | ||
Revision as of 08:33, 9 October 2021
AKTK tag-his tag-2*Mfp5 CDS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 46
Illegal XhoI site found at 514 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a complete CDS for 2 repeats of Mfp5 protein expression. However, the start codon and stop codon is not contained in the sequence. So please add the start codon and the stop codon to the beginning and the end of the sequence before you put this part into the protein expression plasmid(which we recommend pET28).
Source
From literature of Eugene Kim's team from Washington University in St. Louis.