Difference between revisions of "Part:BBa K3726080"

Latest revision as of 23:07, 21 October 2021

P_PrbcL-m36

This part corresponds to a strong constitutive promoter for cyanobacteria. The promoter sequence corresponds with a modified version of the native PCC11801 phycocyanin β-1 subunit promoter.

Despite being a cyanobacterial promoter, it has shown high expression levels also in E. Coli.

The promoter was firstly documented by P. Wangikar et al. ("A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria", ACS Publications, 2021. [Online]. Available: https://doi.org/10.1021/acssynbio.0c00152.), obtained via the screening of a library of promoter variants created by error-prone PCR.

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.

Contribution from MADRID_UCM

Group: MADRID_UCM (4C_Fuels Project) Author: Jorge Fernández, Alvaro Luego, Santiago Barragán Ariza, Lucia Tapia Peña. Summary: Promoter Prbcl-m36 have demonstrated one of the highest expression levels in Synechococcus elongatus genre as well as related cyanobacteria species. This promoter element has been adapted to Phytobricks standard and succesfully assembled.

This phytobrick compatible promoter is part of a wider collection of regulatory elements for cyanobacteria, which are part of MADRID_UCM 2021 team contribution to iGEM community.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 225
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 225
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 225
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 225
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001.
+<b> Contribution from MADRID_UCM </b>
+Group: MADRID_UCM (4C_Fuels Project)
+Author: Jorge Fernández, Alvaro Luego, Santiago Barragán Ariza, Lucia Tapia Peña.
+Summary: Promoter Prbcl-m36 have demonstrated one of the highest expression levels in <i>Synechococcus elongatus genre </i> as well as related cyanobacteria species. This promoter element has been adapted to Phytobricks standard and succesfully assembled.
+This phytobrick compatible promoter is part of a wider collection of  regulatory elements for cyanobacteria, which are part of MADRID_UCM 2021 team contribution to iGEM community.