Difference between revisions of "Part:BBa K3726088:Design"
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===Design Notes===
 
===Design Notes===
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The RBS has been calculated using DeNovo DNA Software. Sequence has been designing including the resulting MoClo Overhangs after level 1 assembly. In accordance with the design of Marburg Collection https://2018.igem.org/Team:Marburg/Part_Collection, the bases TACTAGAG  in the 5’ end, and the bases TAATCAATG are part of the RBS sequence.  
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The RBS has been calculated using DeNovo DNA Software. Sequence has been designing including the resulting MoClo Overhangs after level 1 assembly. In accordance with the design of Marburg Collection https://2018.igem.org/Team:Marburg/Part_Collection, the bases TACTAGAG  in the 5’ end, and the bases TAATCAATG are part of the RBS sequence.
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===Source===
 
===Source===

Revision as of 17:36, 8 October 2021


RBS_NphT7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS has been calculated using DeNovo DNA Software. Sequence has been designing including the resulting MoClo Overhangs after level 1 assembly. In accordance with the design of Marburg Collection https://2018.igem.org/Team:Marburg/Part_Collection, the bases TACTAGAG in the 5’ end, and the bases TAATCAATG are part of the RBS sequence.

Source

Coding sequence of this RBS has been calculated using DeNovo DNA Software. To know more about Marburg collection guidelines visit https://2018.igem.org/Team:Marburg/Part_Collection


References

A. Reis and H. Salis, "An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models", ACS Synthetic Biology, vol. 9, no. 11, pp. 3145-3156, 2020.