Difference between revisions of "Part:BBa K3930029"

 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3930029 SequenceAndFeatures</partinfo>
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The promoter Trc-Theo is an inducible promoter upon addition of IPTG into the media of culture. moreover there is a translation control due to riboswitch which can be activated by adding theophyline in the media of culture.  
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<h2>Introduction</h2>
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<p>The promoter Ptrc-riboswitch E comes from the design of the publication Nakahira et al. 2013. It corresponds to a double regulation of the downstream gene expression. The first is transcriptional regulation by the IPTG-inducible TRC promoter. Finally, the second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the rbs essential for mRNA translation. This promoter has been described for a cyanobacterium Synechococcus elongatus PCC 7942 control of a gene expression.</p>
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<h2>References</h2>
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    <li>Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. 2013. Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942. Plant and Cell Physiology. 54(10):1724–1735. doi:10.1093/pcp/pct115.</li>
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<span class='h3bb'>Sequence and Features</span>
 
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Revision as of 20:26, 12 October 2021


IPTG and theophylline inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

The promoter Ptrc-riboswitch E comes from the design of the publication Nakahira et al. 2013. It corresponds to a double regulation of the downstream gene expression. The first is transcriptional regulation by the IPTG-inducible TRC promoter. Finally, the second regulation is post-transcriptional, as a theophylline-inducible aptamer sequesters the rbs essential for mRNA translation. This promoter has been described for a cyanobacterium Synechococcus elongatus PCC 7942 control of a gene expression.


References

  1. Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. 2013. Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942. Plant and Cell Physiology. 54(10):1724–1735. doi:10.1093/pcp/pct115.
    2.