Difference between revisions of "Part:BBa K3970012"
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==Usage and Biology== | ==Usage and Biology== | ||
TDH3 is our core promoter, we find that TDH3 has the strongest expression in our system. In order to establish DNA modules with predictable properties for the assembly of the general transcription initiation complex in S. cerevisiae, the article selected six S. cerevisiae promoters which are commonly used for heterologous gene expression in this host: GAL1p, PDC1p, TPI1p, TDH3p, ENO1p, and PGK1p. And finally found that TDH3 has the strongest expression. | TDH3 is our core promoter, we find that TDH3 has the strongest expression in our system. In order to establish DNA modules with predictable properties for the assembly of the general transcription initiation complex in S. cerevisiae, the article selected six S. cerevisiae promoters which are commonly used for heterologous gene expression in this host: GAL1p, PDC1p, TPI1p, TDH3p, ENO1p, and PGK1p. And finally found that TDH3 has the strongest expression. | ||
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Latest revision as of 12:32, 8 October 2021
cpTDH3
Usage and Biology
TDH3 is our core promoter, we find that TDH3 has the strongest expression in our system. In order to establish DNA modules with predictable properties for the assembly of the general transcription initiation complex in S. cerevisiae, the article selected six S. cerevisiae promoters which are commonly used for heterologous gene expression in this host: GAL1p, PDC1p, TPI1p, TDH3p, ENO1p, and PGK1p. And finally found that TDH3 has the strongest expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]