Difference between revisions of "Part:BBa K3726104"

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This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 1 of PCC11801.  
 
This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 1 of PCC11801.  
  
Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the  level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection. (Referencia a Marburg).  
+
Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the  level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection: https://2019.igem.org/Team:Marburg/Part_Collection.  
  
 
Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector / 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.
 
Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector / 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.

Revision as of 14:05, 8 October 2021


5CON1(H)_NS1(mod)-up (PCC 11801)


This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 1 of PCC11801.

Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection: https://2019.igem.org/Team:Marburg/Part_Collection.

Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector / 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.

The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit.


Sequence & Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
    Illegal AgeI site found at 594
  • 1000
    COMPATIBLE WITH RFC[1000]