Difference between revisions of "Part:BBa K3970000"
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<partinfo>BBa_K3970000 short</partinfo>
 
<partinfo>BBa_K3970000 short</partinfo>
  
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==Usage and Biology==
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Ampicillin resistance genes are the most widely used selective markers in gene manipulation, and most plasmid vectors cloned in E. coli carry this gene.  Penicillin inhibits the synthesis of cell wall peptidoglycan, binds to and inhibits the activity of related enzymes, and inhibits the peptide transfer reaction.  The ampicillin resistance gene encodes an enzyme that is secreted into the periplasmic region of the bacteria, inhibiting the peptide transfer reaction and catalyzing β -lactam ring hydrolysis, thereby detoxicating ampicillin.Penicillin is a general term for a class of compounds, its molecular structure is composed of side chain R-CO- and main nuclear 6-amino penicilanic acid two parts.  There is A saturated thiazole ring (A) and A β -lactam ring in 6-APA+.6-APA is A dipeptide formed by the condensation of L-hemi-dephosphoric acid and valine.
  
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==Sequence==
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<html><h3 style="font-size: 25px;font-weight: 700;">1.Usage and Biology</h3></html>
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<html><p style="font-size: 14px;word-wrap:break-word;">Atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa</p><br><br></html>
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<html><p style="font-size: 18px;">Ampicillin resistance genes are the most widely used selective markers in gene manipulation, and most plasmid vectors cloned in E. coli carry this gene.  Penicillin inhibits the synthesis of cell wall peptidoglycan, binds to and inhibits the activity of related enzymes, and inhibits the peptide transfer reaction.  The ampicillin resistance gene encodes an enzyme that is secreted into the periplasmic region of the bacteria, inhibiting the peptide transfer reaction and catalyzing β -lactam ring hydrolysis, thereby detoxicating ampicillin.Penicillin is a general term for a class of compounds, its molecular structure is composed of side chain R-CO- and main nuclear 6-amino penicilanic acid two parts.  There is A saturated thiazole ring (A) and A β -lactam ring in 6-APA+.6-APA is A dipeptide formed by the condensation of L-hemi-dephosphoric acid and valine.</p></html>
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<html><h3 style="font-size: 25px;font-weight: 700;">2.sequence</h3></html>
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<html><p style="font-size: 14px;word-wrap:break-word;">Atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa</p></html>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3970000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3970000 SequenceAndFeatures</partinfo>

Revision as of 02:47, 7 October 2021


AmpR

Usage and Biology

Ampicillin resistance genes are the most widely used selective markers in gene manipulation, and most plasmid vectors cloned in E. coli carry this gene.  Penicillin inhibits the synthesis of cell wall peptidoglycan, binds to and inhibits the activity of related enzymes, and inhibits the peptide transfer reaction.  The ampicillin resistance gene encodes an enzyme that is secreted into the periplasmic region of the bacteria, inhibiting the peptide transfer reaction and catalyzing β -lactam ring hydrolysis, thereby detoxicating ampicillin.Penicillin is a general term for a class of compounds, its molecular structure is composed of side chain R-CO- and main nuclear 6-amino penicilanic acid two parts.  There is A saturated thiazole ring (A) and A β -lactam ring in 6-APA+.6-APA is A dipeptide formed by the condensation of L-hemi-dephosphoric acid and valine.

Sequence

Atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]