Difference between revisions of "Part:BBa K4047021:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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This terminator was able to successfully allow transcription and expression of our selective antibiotic marker kanamycin, allowing transformed colonies to grow on a kanamycin-containing agar plate. This antibiotic resistance marker, with its corresponding promoter and terminator, was used in the plasmid <partinfo>BBa_K4047039</partinfo>. The insertion of the part both disrupted functional gene expression of our target gene and served as a selective marker for successful integration. This part was successfully used in the 2021 MiamiU_OH team project, with a picture shown below of the resulting growth as experimental evidence.
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how you used this part and how it worked out.
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[[File:Kanamycin Resistance in Synechococcus Experience.png|200px|thumb|left|Growth of Synechococcus elongatus PCC 7942 on kanamycin-containing plate after transformation with plasmids containing the kanamycin resistance part BBa_K4047019]]
  
 
===Applications of BBa_K4047021===
 
===Applications of BBa_K4047021===
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This part can be used in plasmids to allow functional transcription and expression of the selective marker kanamycin, indicating when colonies have been successfully transformed.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 21:01, 20 October 2021


This terminator was able to successfully allow transcription and expression of our selective antibiotic marker kanamycin, allowing transformed colonies to grow on a kanamycin-containing agar plate. This antibiotic resistance marker, with its corresponding promoter and terminator, was used in the plasmid BBa_K4047039. The insertion of the part both disrupted functional gene expression of our target gene and served as a selective marker for successful integration. This part was successfully used in the 2021 MiamiU_OH team project, with a picture shown below of the resulting growth as experimental evidence.

Growth of Synechococcus elongatus PCC 7942 on kanamycin-containing plate after transformation with plasmids containing the kanamycin resistance part BBa_K4047019

Applications of BBa_K4047021

This part can be used in plasmids to allow functional transcription and expression of the selective marker kanamycin, indicating when colonies have been successfully transformed.

User Reviews

UNIQcb7f3214d2198a22-partinfo-00000001-QINU UNIQcb7f3214d2198a22-partinfo-00000002-QINU