Difference between revisions of "Part:BBa K3726002:Design"
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===Source=== | ===Source=== | ||
| − | Coding sequence of this Crotonyl-CoA reductase has been found within the genome of Streptomyces Collinus. | + | Coding sequence of this Crotonyl-CoA reductase has been found within the genome of <i>Streptomyces Collinus</i>. |
Uniprot reference: https://www.uniprot.org/uniprot/Q53865 | Uniprot reference: https://www.uniprot.org/uniprot/Q53865 | ||
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===References=== | ===References=== | ||
"Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true. | "Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true. | ||
Revision as of 13:23, 5 October 2021
CDS_Ccr
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 85
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 85
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 168
Illegal BamHI site found at 507 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 85
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 85
Illegal NgoMIV site found at 763
Illegal AgeI site found at 291 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part corresponds with the codon optimized coding sequence of Ccr. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases
Source
Coding sequence of this Crotonyl-CoA reductase has been found within the genome of Streptomyces Collinus. Uniprot reference: https://www.uniprot.org/uniprot/Q53865
References
"Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true.