Difference between revisions of "Part:BBa K3726005:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
This part corresponds with the codon optimized coding sequence of Slr1192 enzyme.
 
This part corresponds with the codon optimized coding sequence of Slr1192 enzyme.
−
Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in < Synechococcus elongatus PCC7942 >.
+
Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <Synechococcus elongatus PCC7942/>.
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
  

Revision as of 17:22, 4 October 2021


CDS_Slr1192


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
    Illegal BglII site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of Slr1192 enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <Synechococcus elongatus PCC7942/>. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Aldehyde reductase has been found within the genome of Synechocystis PC 6803. Uniprot reference: https://www.uniprot.org/uniprot/P74721


References

R. Miao, X. Liu, E. Englund, P. Lindberg and P. Lindblad, "Isobutanol production in Synechocystis PCC 6803 using heterologous and endogenous alcohol dehydrogenases", 2021.