Difference between revisions of "Part:BBa K3726002:Design"

Revision as of 13:22, 5 October 2021

CDS_Ccr

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 85
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 85
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 168
Illegal BamHI site found at 507
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 85
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 85
Illegal NgoMIV site found at 763
Illegal AgeI site found at 291
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part corresponds with the codon optimized coding sequence of Ccr. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Crotonyl-CoA reductase has been found within the genome of Streptomyces Collinus. Uniprot reference: https://www.uniprot.org/uniprot/Q53865

References

"Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true.

@@ Line 8: / Line 8: @@
 ===Design Notes===
 This part corresponds with the codon optimized coding sequence of Ccr.
-Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.
+Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>. In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases