Difference between revisions of "Part:BBa K3711053"
Line 3: | Line 3: | ||
<partinfo>BBa_K3711053 short</partinfo> | <partinfo>BBa_K3711053 short</partinfo> | ||
− | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 17: | Line 17: | ||
<partinfo>BBa_K3711053 parameters</partinfo> | <partinfo>BBa_K3711053 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | <h1>'''Description'''</h1> | ||
+ | This is a composite part for extracellular expression of ROX1. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, the signal peptide, α-factor , is used to express ROX1 outside of the cell. | ||
+ | <h1>'''Usage and Biology'''</h1> | ||
+ | A kind of yeast protein, which could inhibit promoter Panb1 and then silence following genes of it. | ||
+ | <h1>'''Molecular cloning'''</h1> | ||
+ | ***tu*** |
Revision as of 08:45, 21 October 2021
Pynr071c-α factor-ROX1-AOX1 Terminator
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2711
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1438
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1630
Illegal SapI site found at 1889
Description
This is a composite part for extracellular expression of ROX1. Pynr071c is the promoter of Aldose 1-isomerase superfamily protein YNR071C. In the presence of xylose, the signal peptide, α-factor , is used to express ROX1 outside of the cell.
Usage and Biology
A kind of yeast protein, which could inhibit promoter Panb1 and then silence following genes of it.
Molecular cloning
- tu***