Difference between revisions of "Part:BBa K3882001"

Revision as of 03:24, 5 October 2021

E. bsuahlscout-QS-bsuahlteminator

Our E. bsuahlscout bio-brick contains 3 key elements including LuxR, QS promoter, and mCherry. LUXR is a protein coded by LuxR gene. The LuxR gene is regulated by T7 promoter, Lac operator and T7 terminator. The LUXR protein can combine the AHL to formate a LUXR-AHL complex which can bind to the QS promoter and recruit RNA polymerase to start the down-stream gene expression. QS promoter is a DNA element to react with LUXR-AHL complex. The mCherry gene is down-stream report gene of QS promoter and codes a red fluorescence protein.

Our E. bsuahlteminator bio-brick contains 2 key elements including pvdq and eGFP. PVDQ is a protein coded by pvdq gene. The pvdq gene is regulated by T7 promoter, Lac operator and T7 terminator. The PVDQ protein can catalyze the deacylation of acyl-homoserine lactone (AHL) which can prevent the activation of LUXR by eliminating the AHL in the environment. In order to increase the stability and show the production quality in a real time manner. We use a protein linker and 3x HA tag to link the PVDQ protein with eGFP. We also use 3xHis tag at the c-terminal of eGFP to increase the efficiency to purify the recombination proteins. Our results shows the great stability in solutions and almost no aggregation happens after elusion.

Using QS promoter to start the E. bsuahlteminator bio-brick, we are able to detecting the AHL in the environment and do the clearance at the same time.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3882001 short</partinfo>
-Part1 equipped with LuxR to sense and combine the autoinducer, N-Acyl Homoserine Lactone (AHL) in the environment. After that, LuxR-AHL complex will be activated and bind to luxr-AHL response QS element to initiate the expression of mcherry report gene. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. LuxR functions as receiving AHL signal molecules, and activates mCherry red fluorescence gene. QS promoter is the place that LuxR can recruit RNA polymerase. mCherry is a red fluorescence reporting gene.
+Our E. bsuahlscout bio-brick contains 3 key elements including LuxR, QS promoter, and mCherry. LUXR is a protein coded by LuxR gene. The LuxR gene is regulated by T7 promoter, Lac operator and T7 terminator. The LUXR protein can combine the AHL to formate a LUXR-AHL complex which can bind to the QS promoter and recruit RNA polymerase to start the down-stream gene expression. QS promoter is a DNA element to react with LUXR-AHL complex. The mCherry gene is down-stream report gene of QS promoter and codes a red fluorescence protein.
+Our E. bsuahlteminator bio-brick contains 2 key elements including pvdq and eGFP. PVDQ is a protein coded by pvdq gene. The pvdq gene is regulated by T7 promoter, Lac operator and T7 terminator. The PVDQ protein can catalyze the deacylation of acyl-homoserine lactone (AHL) which can prevent the activation of LUXR by eliminating the AHL in the environment. In order to increase the stability and show the production quality in a real time manner. We use a protein linker and 3x HA tag to link the PVDQ protein with eGFP. We also use 3xHis tag at the c-terminal of eGFP to increase the efficiency to purify the recombination proteins. Our results shows the great stability in solutions and almost no aggregation happens after elusion.
+Using QS promoter to start the E. bsuahlteminator bio-brick, we are able to detecting the AHL in the environment and do the clearance at the same time.
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