Difference between revisions of "Part:BBa K3814076"
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| − | + | We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli. | |
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| + | To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes. | ||
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| + | We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here. | ||
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Latest revision as of 00:40, 22 October 2021
Cluster 8
We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.
To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.
We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4578
Illegal NheI site found at 4601 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4975
Illegal XhoI site found at 75 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]