Difference between revisions of "Part:BBa K3814050"

 
 
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<partinfo>BBa_K3814050 short</partinfo>
 
<partinfo>BBa_K3814050 short</partinfo>
  
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fliK downstream homology region, gene clusters will be recombineered into the fliK gene in E. coli.
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This 50bp section of DNA is from the fliK gene that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome.
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This is a region of the fliK gene, and gene clusters will be recombineered into it in E. coli.
  
 
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Latest revision as of 15:03, 21 October 2021


fliK downstream homology region

This 50bp section of DNA is from the fliK gene that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome.

This is a region of the fliK gene, and gene clusters will be recombineered into it in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]