Difference between revisions of "Part:BBa K3861018"

 
 
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<partinfo>BBa_K3861018 short</partinfo>
 
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Part is composed of the basic parts <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861011">BBa_K3861011</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861013">BBa_K3861013</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861015">BBa_K3861015</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861001">BBa_K3861001</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861016">BBa_K3861016</a>.
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The part is thought to be used for the display of the recombinant lectin domain rBC2LCN of <I>Burkholderia cenocepacia</I> on the surface of <I>Salmonella</I> minicells. Expression is aimed to be driven by the anhydrotetracycline-inducible tet promoter. AIDA-I autotransporter is required for the export of the lectin and its display on the bacterial surface since AIDA-I is an adhesion located on the bacterial cell surface.
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<partinfo>BBa_K3861018 parameters</partinfo>
 
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==='''References'''===
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1. Yu, H. et al. Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells, <I>ACS Synthetic Biology</I>, (2021).
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Latest revision as of 03:14, 22 October 2021


tetR-Ptet-AIDAsignal-HA-rBC2LCN-AIDAautotransporter

Part is composed of the basic parts BBa_K3861011, BBa_K3861013, BBa_K3861015, BBa_K3861001 and BBa_K3861016. The part is thought to be used for the display of the recombinant lectin domain rBC2LCN of Burkholderia cenocepacia on the surface of Salmonella minicells. Expression is aimed to be driven by the anhydrotetracycline-inducible tet promoter. AIDA-I autotransporter is required for the export of the lectin and its display on the bacterial surface since AIDA-I is an adhesion located on the bacterial cell surface.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1397
    Illegal BamHI site found at 2180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 737


References

1. Yu, H. et al. Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells, ACS Synthetic Biology, (2021).