Difference between revisions of "Part:BBa K4074048:Design"

 
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===Source===
 
===Source===
  
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-
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pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab
  
 
===References===
 
===References===

Latest revision as of 03:31, 21 October 2021


pSB_THRC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal XbaI site found at 2205
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
    Illegal NotI site found at 3520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal BamHI site found at 2211
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal XbaI site found at 2205
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal XbaI site found at 2205
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

-


Source

pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab

References