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TetR is a kind of protein that can find and combined with TRE DNA structure domain.
 
TetR is a kind of protein that can find and combined with TRE DNA structure domain.
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                TetR
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                <p>TetR can be identified and combined with TRE, which is an important part of the Tet-off system. In the absence of tetracycline, TetR binds to TRE. When tetracycline exists, the binding of TetR and TRE will be blocked and the Tet-off system will be shut down.
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                1.Pattern diagram
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                <img class="image" src="https://2021.igem.org/wiki/images/d/d1/T--CSU_CHINA--tupian13.png">
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram TetR-ELK</p>
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                2.Experiment
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                2.1 Method
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                <p>After TetR binds to TRE, phosphorylation ELK activates downstream reporting gene mCherry expression, and we observe the expression with laser confocal microscope.
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                2.2 Results
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                <img class="image" src="https://2021.igem.org/wiki/images/5/54/T--CSU_CHINA--tupian14.png">
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p>
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                3.Caution
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                <p>If the green fluorescent protein is expressed too much, and if the green fluorescence is too strong, it may cause a series of colors. In order to ensure the accuracy of the experimental results, the level of noise reduction should be raised.
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                Reference：
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                <p>[1]Haifeng Ye, Mingqi Xie, Shuai Xue.Self-adjusting synthetic gene circuit for correcting insulin resistance[J].Nat Biomed Eng.2017 Jan;1(1):0005.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:50, 20 October 2021


TetR

TetR is a kind of protein that can find and combined with TRE DNA structure domain.

TetR

TetR can be identified and combined with TRE, which is an important part of the Tet-off system. In the absence of tetracycline, TetR binds to TRE. When tetracycline exists, the binding of TetR and TRE will be blocked and the Tet-off system will be shut down.

1.Pattern diagram

Fig.1 The model diagram TetR-ELK

2.Experiment

2.1 Method

After TetR binds to TRE, phosphorylation ELK activates downstream reporting gene mCherry expression, and we observe the expression with laser confocal microscope.

2.2 Results

Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system

3.Caution

If the green fluorescent protein is expressed too much, and if the green fluorescence is too strong, it may cause a series of colors. In order to ensure the accuracy of the experimental results, the level of noise reduction should be raised.

Reference：

[1]Haifeng Ye, Mingqi Xie, Shuai Xue.Self-adjusting synthetic gene circuit for correcting insulin resistance[J].Nat Biomed Eng.2017 Jan;1(1):0005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]