Difference between revisions of "Part:BBa K3817008:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We purposely designed the primers by NCBI and our method in order for them to bind specifically, and finally ensured that the difference in annealing temperature Tm between the | + | We purposely designed the primers by NCBI and our method in order for them to bind specifically, and finally ensured that the difference in annealing temperature Tm between the forward and reverse primers was extremely small. |
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===Source=== | ===Source=== | ||
Latest revision as of 15:36, 1 October 2021
Pbluescript Ⅱ SK+ MCS forward primer
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We purposely designed the primers by NCBI and our method in order for them to bind specifically, and finally ensured that the difference in annealing temperature Tm between the forward and reverse primers was extremely small.
Source
We design this primer.