Difference between revisions of "Part:BBa K3982001"

Revision as of 15:44, 12 October 2021

Cas14a1 expression gene

This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by Team IISER_Berhampur 2021 in Project CODE M.

Usage and Biology

Cas14a1 (also called Un1Cas12f1) is a miniature cas protein used in CRISPR/Cas technology identified from archaebacteria and bacteriophages. It is a RNA guided nuclease that cleaves ssDNA without any Protospacer Adjacent Motif (PAM) specificity i.e. PAM independent ssDNA cleavage. This enables high-fidelity Single-nucleotide polymorphism (SNP) genotyping. It is guided by a single guide RNA (sgRNA) which has two components - CRISPR RNA (crRNA) and Trans-activating crispr RNA (tracrRNA).

Click here to know more about how this part is used in project CODE M by Team IISER_Berhampur 2021.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1357
Illegal BamHI site found at 951
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1357
Illegal PstI site found at 147
Illegal PstI site found at 313
Illegal AgeI site found at 607
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3982001 short</partinfo>
-It encodes the Cas14a1 protein. It is a compact RNA guided nuclease.
+This sequence encodes the Cas14a1 protein. It is a highly compact RNA guided nuclease used by [https://2021.igem.org/Team:IISER_Berhampur Team IISER_Berhampur 2021] in Project CODE M.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Cas14a1 (also called Un1Cas12f1) is a miniature cas protein used in CRISPR/Cas technology identified from archaebacteria and bacteriophages. It is a RNA guided nuclease that cleaves ssDNA without any Protospacer Adjacent Motif (PAM) specificity i.e. PAM independent ssDNA cleavage. This enables high-fidelity Single-nucleotide polymorphism (SNP) genotyping. It is guided by a single guide RNA (sgRNA) which has two components - CRISPR RNA (crRNA) and Trans-activating crispr RNA (tracrRNA).
+ [https://2021.igem.org/Team:IISER_Berhampur Click here] to know more about how this part is used in project CODE M by Team IISER_Berhampur 2021.
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