Difference between revisions of "Part:BBa K3771034"

 
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<br><b style="font-size:1.3rem">Description</b>
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<br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.
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<br><b style="font-size:1.3rem">Biology</b>
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<br>The <i>ompA</i> promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria[1]. Signal transduction from the outer membrane to the inner membrane activates the <i>pspA</i> promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.
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<br><b style="font-size:1.3rem">Usage</b>
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<br>We ligased the NPO-OmpA* fragment and pspA-CoaBC on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
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<br><b style="font-size:1.3rem">Characterization</b>
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<br>Double digestion results are shown in Figure 1.
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<img src="圖片網址" style="width:35%;">
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<p align="center">圖片描述</p>
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<br><b style="font-size:1.3rem">References</b>
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<br>1. Darwin AJ. The phage-shock-protein response. <i>Molecular Microbiology</i>. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x
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<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 20:03, 17 October 2021


PpspA-CoaBC-PompA-OmpA/OprF


Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.

Biology

The ompA promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria[1]. Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.

Usage

We ligased the NPO-OmpA* fragment and pspA-CoaBC on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.

Characterization

Double digestion results are shown in Figure 1.

圖片描述


References

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal BamHI site found at 2515
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]