Difference between revisions of "Part:BBa K3771022"
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+ | <br><b style="font-size:1.3rem">Description</b> | ||
+ | <br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC. <br> | ||
+ | |||
+ | |||
+ | <br><b style="font-size:1.3rem">Biology</b> | ||
+ | <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/ | ||
+ | " alt="" target="_blank">[3]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway. | ||
+ | <br> | ||
+ | |||
+ | <br><b style="font-size:1.3rem">Usage</b> | ||
+ | <br>We ligased the <i>coaBC</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. <br> | ||
+ | |||
+ | <br><b style="font-size:1.3rem">Characterization</b> | ||
+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/wiki/images/c/c5/T--NCKU_Tainan--colony_pcr_pspA_CoaBC.jpg | ||
+ | l | ||
+ | " style="width:35%;"> | ||
+ | </div> | ||
+ | <p align="center">圖片描述</p> | ||
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+ | <br><b style="font-size:1.3rem">Reference</b> | ||
+ | <br>3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a><br> | ||
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Revision as of 12:52, 17 October 2021
PpspA-CoaBC
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [3] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.
Usage
We ligased the coaBC fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
Characterization
![](https://2021.igem.org/wiki/images/c/c5/T--NCKU_Tainan--colony_pcr_pspA_CoaBC.jpg
l
)
圖片描述
Reference
3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features