Difference between revisions of "Part:BBa K3771021"

 
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<partinfo>BBa_K3771021 short</partinfo>
 
<partinfo>BBa_K3771021 short</partinfo>
  
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<br><b style="font-size:1.3rem">Description</b>
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  <br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU.
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  <br><b style="font-size:1.3rem">Biology</b>
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  <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">[3]</a> Signal transduction from the outer membrane to the inner membrane activates the <i>pspA</i> promoter, initiating expression of JJU. JJU converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
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<br><b style="font-size:1.3rem">Usage</b>
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  <br>We ligased the <i>jju</i>-6xHis fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. The 6xhis allows for confirmation of JJU expression by western blot using the anti-6xHis antibody. <br>
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    <br><b style="font-size:1.3rem">Characterization</b>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/0/08/T--NCKU_Tainan--colony_pcr_pspA_JJU10_6xHis.jpg
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" style="width:35%;">
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<p align="center">圖片描述</p>
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  <br><b style="font-size:1.3rem">Reference</b>
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  <br>3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x
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<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 12:45, 17 October 2021


PpspA-JJU10-6xHis


Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU.

Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [3] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU. JJU converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.

Usage
We ligased the jju-6xHis fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. The 6xhis allows for confirmation of JJU expression by western blot using the anti-6xHis antibody.

Characterization

圖片描述


Reference
3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features BBa_K3771021 SequenceAndFeatures