Difference between revisions of "Part:BBa K415202"
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| − | [https://2021.igem.org/Team:Thessaly Thessaly 2021]contributed to the characterization of this part by adding new documentation learned from the literature | + | [https://2021.igem.org/Team:Thessaly Thessaly 2021]contributed to the characterization of this part by adding new documentation learned from the literature highlighting why CymR is a better repressor for the cumate kill-switch. |
===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K415202 SequenceAndFeatures</partinfo> | <partinfo>BBa_K415202 SequenceAndFeatures</partinfo> | ||
Revision as of 10:01, 2 October 2021
cymR Repressor Protein
The cymR repressor tightly and efficiently binds the cmt operator. In conjunction with part BBa_K415200, this repressor may be used to create an inverter or similar logical circuitry. cymR changes conformation upon induction with cumate (isopropylbenozate), and may be used in a manner similar to the lacI/IPTG system.
The cymR repressor appears to bind the complementary cmt operator more efficiently than lac repressor binds the lac operator, according to Choi, et al. in a recent (as of 2010) paper titled, "A novel, versatile, and tightly regulated expression system for E. Coli strains."
Thessaly 2021 Literature Characterization
Thessaly 2021contributed to the characterization of this part by adding new documentation learned from the literature highlighting why CymR is a better repressor for the cumate kill-switch.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 357
Illegal XhoI site found at 138 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 40