Difference between revisions of "Part:BBa K3852004"
 
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<partinfo>BBa_K3852004 short</partinfo>
 
<partinfo>BBa_K3852004 short</partinfo>
  
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TAS2R46 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway.
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This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception.
  
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After being stimulated by bitter substances, the bitter receptors activate -gustducin and effector enzyme PDE, and reduce the concentration of intracellular second messenger called ring nucleoside acid(cNMP). After that, calcium channels in the cell membrane were opened to make extracellular calcium ions flow in, and the concentration of intracellular calcium ions increased. Finally, the membrane depolarizes, releasing neurotransmitters.In our experiments, we used this gene to express bitter taste receptors in Saccharomyces cerevisiae.
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Several papers have shown that bitter substances are recognized by the bitter receptor TAS2R, a family of G protein-coupled receptors expressed in taste tissue. In humans, more than 25 TAS2Rs have been identified; some of these are activated by bitter substances and function as bitter taste receptors. Because many bitter tastants activate hTAS2R, it has been thought that humans perceive bitterness via those receptors. Experiments show that a variety of bitter peptides can activate T2R4.In our experiments, we used this gene to express bitter taste receptors in Saccharomyces cerevisiae.We acquire it from synthesis company.
  
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===Usage and Biology===
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====Usage and Biology====
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Gustducin-coupled receptor for denatonium and N6-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. In airway epithelial cells, binding of denatonium increases the intracellular calcium ion concentration and stimulates ciliary beat frequency.
  
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Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 (By similarity).
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====Reference====
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[1] Mueller, K., Hoon, M., Erlenbach, I. et al. Erratum: The receptors and coding logic for bitter taste. [J]Nature 446, 342 (2007).
  
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In airway epithelial cells, binding of bitter compounds increases the intracellular calcium ion concentration and stimulates ciliary beat frequency (By similarity).We acquire it from synthesis company.
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[2] Kenji Maehashi, Mami Matano, Hong Wang et al.Bitter peptides activate hTAS2Rs, the human bitter receptors[J]Biochemical and Biophysical Research Communications.2008,365(4): 851-855.
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===Reference===
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[1] Lindemann B. Receptors and transduction in taste[J]Nature,2001,413(6852):219-225.
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Latest revision as of 12:46, 18 October 2021


T2R4

This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception.

Several papers have shown that bitter substances are recognized by the bitter receptor TAS2R, a family of G protein-coupled receptors expressed in taste tissue. In humans, more than 25 TAS2Rs have been identified; some of these are activated by bitter substances and function as bitter taste receptors. Because many bitter tastants activate hTAS2R, it has been thought that humans perceive bitterness via those receptors. Experiments show that a variety of bitter peptides can activate T2R4.In our experiments, we used this gene to express bitter taste receptors in Saccharomyces cerevisiae.We acquire it from synthesis company.

Usage and Biology

Gustducin-coupled receptor for denatonium and N6-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. In airway epithelial cells, binding of denatonium increases the intracellular calcium ion concentration and stimulates ciliary beat frequency.

Reference

[1] Mueller, K., Hoon, M., Erlenbach, I. et al. Erratum: The receptors and coding logic for bitter taste. [J]Nature 446, 342 (2007).

[2] Kenji Maehashi, Mami Matano, Hong Wang et al.Bitter peptides activate hTAS2Rs, the human bitter receptors[J]Biochemical and Biophysical Research Communications.2008,365(4): 851-855.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]