Difference between revisions of "Part:BBa K3794000:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12  
+
Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12.
 +
 
 +
Added N-terminal 6xHis tag with start codon and TEV cleavage site for protein purification via Ni-NTA column
  
  
Line 14: Line 16:
 
===Source===
 
===Source===
  
−
This part was synthesised by IDT. The DNA sequence was obtained from UniProtKB: U5Y3S6, and codon optimised for expression in E.Coli
+
This part was synthesised as a composite by IDT (with an upstream LacI regulatory (BBa_R001). The protein sequence was obtained from UniProtKB: U5Y3S6, a 6xHis tag and TEV cleavage site was added, and codon optimised for expression in E.Coli K12
  
 
===References===
 
===References===

Revision as of 18:41, 19 October 2021


PVFP-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12.

Added N-terminal 6xHis tag with start codon and TEV cleavage site for protein purification via Ni-NTA column



Source

This part was synthesised as a composite by IDT (with an upstream LacI regulatory (BBa_R001). The protein sequence was obtained from UniProtKB: U5Y3S6, a 6xHis tag and TEV cleavage site was added, and codon optimised for expression in E.Coli K12

References