Difference between revisions of "Part:BBa K3739083"
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<partinfo>BBa_K3739083 short</partinfo>
 
<partinfo>BBa_K3739083 short</partinfo>
  
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Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. LMT is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant.  
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Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. LMT is a signal peptile. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.  
  
 
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Revision as of 17:18, 20 October 2021


LMT-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. LMT is a signal peptile. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 655
    Illegal NgoMIV site found at 1390
    Illegal NgoMIV site found at 1626
    Illegal AgeI site found at 292
    Illegal AgeI site found at 1119
    Illegal AgeI site found at 1615
  • 1000
    COMPATIBLE WITH RFC[1000]