Difference between revisions of "Part:BBa K4060106:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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====Assembly of PreB3 (pSB1C3)====
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how you used this part and how it worked out.
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The gene fragment Pre B3 is composed of Pre A1 and Pre A3. We digested the vector fragment Pre A1 with SpeI and PstI and the insert fragment Pre A3 with XbaI and PstI. We ligated these fragments together to form Pre B3.
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<html><body>
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<img src="https://2021.igem.org/wiki/images/c/c5/T--NYCU-Taipei--PreB3.png" height="475px" width="750px"/>
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</body></html>
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Fig. 1 Agarose electrophoresis in order to verify Pre B3 (Construct 3). Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3,4 Pre B3 digested with BamHI and HindIII.
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====1. The RFP expression of Construct 3====
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We used RFP(E1010) to monitor the expression of TetR, so that we can figure out how L-arabinose will influence the expression of TetR in our Design 1.
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<html><body>
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<img src="https://2021.igem.org/wiki/images/c/c5/T--NYCU-Taipei--PreB3.png" height="475px" width="750px"/>
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</body></html>
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Fig. 2 The RFP intensity of construct 3(Pre B3) from 0 hr~ 24hrs in different L-arabinose concentrations.
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<html><body>
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<img src="https://2021.igem.org/wiki/images/c/c5/T--NYCU-Taipei--PreB3.png" height="475px" width="750px"/>
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</body></html>
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Fig. 3 After 24 hrs cultivation, the RFP intensity of construct 3 in different L-arabinose concentrations
  
 
===Applications of BBa_K4060106===
 
===Applications of BBa_K4060106===

Revision as of 14:29, 7 December 2021


Assembly of PreB3 (pSB1C3)

The gene fragment Pre B3 is composed of Pre A1 and Pre A3. We digested the vector fragment Pre A1 with SpeI and PstI and the insert fragment Pre A3 with XbaI and PstI. We ligated these fragments together to form Pre B3.

Fig. 1 Agarose electrophoresis in order to verify Pre B3 (Construct 3). Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3,4 Pre B3 digested with BamHI and HindIII.

1. The RFP expression of Construct 3

We used RFP(E1010) to monitor the expression of TetR, so that we can figure out how L-arabinose will influence the expression of TetR in our Design 1.

Fig. 2 The RFP intensity of construct 3(Pre B3) from 0 hr~ 24hrs in different L-arabinose concentrations.

Fig. 3 After 24 hrs cultivation, the RFP intensity of construct 3 in different L-arabinose concentrations

Applications of BBa_K4060106

User Reviews

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