Difference between revisions of "Part:BBa K4060105:Experience"
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− | + | ====Assembly of PreB2 (pSB1C3)==== | |
− | + | The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS <partinfo>BBa_K115002</partinfo>. We digested the vector fragment <partinfo>BBa_K115002</partinfo> with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2. | |
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+ | <img src="https://2021.igem.org/wiki/images/e/e1/T--NYCU-Taipei--image4.png" height="475px" width="750px"/> | ||
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+ | Fig.6 Agarose electrophoresis in order to verify Pre B2. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B2 digested with NotI. | ||
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===Applications of BBa_K4060105=== | ===Applications of BBa_K4060105=== |
Revision as of 14:24, 7 December 2021
Assembly of PreB2 (pSB1C3)
The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS BBa_K115002. We digested the vector fragment BBa_K115002 with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.
![](https://2021.igem.org/wiki/images/e/e1/T--NYCU-Taipei--image4.png)
Fig.6 Agarose electrophoresis in order to verify Pre B2. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B2 digested with NotI.
Applications of BBa_K4060105
User Reviews
UNIQ731f59a47c292edf-partinfo-00000003-QINU UNIQ731f59a47c292edf-partinfo-00000004-QINU