Difference between revisions of "Part:BBa K4060104:Experience"
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− | + | ====Assembly of Pre B1 (pSB1C3)==== | |
− | + | The gene fragment Pre B1 is composed of Pre A1 and the TetR gene <partinfo>BBa_C0040</partinfo>. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment <partinfo>BBa_C0040</partinfo> with XbaI and PstI. We ligated these fragments together to form Pre B1. | |
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+ | <img src="https://static.igem.org/mediawiki/parts/f/fb/Flocculation_with_inducers.png" height="475px" width="750px"/> | ||
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+ | Fig.5 Agarose electrophoresis in order to verify Pre B1. | ||
===Applications of BBa_K4060104=== | ===Applications of BBa_K4060104=== |
Revision as of 14:15, 7 December 2021
Assembly of Pre B1 (pSB1C3)
The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.
Fig.5 Agarose electrophoresis in order to verify Pre B1.
Applications of BBa_K4060104
User Reviews
UNIQd1c1c3bbfa6acdbe-partinfo-00000003-QINU UNIQd1c1c3bbfa6acdbe-partinfo-00000004-QINU