Difference between revisions of "Part:BBa K4032005"

Revision as of 09:02, 20 October 2021

α-galactosidase-RFP

This part contains the gene of the α-galactosidase-RFP fusion protein.

Usage and Biology

This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. https://www.uniprot.org/uniprot/P06720

Red fluorescence of RFP is observed under the fluorescence microscope and natural light.

Designs

This part expresses the α-galactosidase-RFP fusion protein.

α-galactosidase is from E.coli K-12 MG1655 and RFP is from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .

We removed the stop codon of alpha-galactosidase and the start codon of RFP and connected the ends.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1905
Illegal AgeI site found at 2017
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4032005 short</partinfo>
-aa
+This part contains the gene of the α-galactosidase-RFP fusion protein.
+===Usage and Biology===
+This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. https://www.uniprot.org/uniprot/P06720
+Red fluorescence of RFP is observed under the fluorescence microscope and natural light.
+===Designs===
+This part expresses the α-galactosidase-RFP fusion protein.
+α-galactosidase is from E.coli K-12 MG1655 and RFP is from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .
+We removed the stop codon of alpha-galactosidase and the start codon of RFP and connected the ends.
 <!-- Add more about the biology of this part here