Difference between revisions of "Part:BBa K3805138"

Revision as of 11:55, 16 October 2021

agrBD This part is an important part of the Agr system

AgrD is a 138 bp sequence located in Staphylococcus aureus and has four different species, but all AgrD sequences include an Asp-Glu motif followed immediately by an AIP sequence, and the amino-terminal portion of AgrD has a conserved Ile-Gly motif. All precursor peptides of AgrD have an amphipathic α-helix fragment, which is responsible for anchoring AgrD to the cytoplasmic membrane. AgrB is a 567bp sequence that transcribes a transmembrane protein with multiple active centres, the topology of which is not well understood. H77 and C84 are essential amino acids for the synthesis of active AIP. It has been suggested that AgrB is a cysteine protease involved in the first step of the AgrD process and produces an acylenzymethioesterintermediate.

Although the information is not clear, it is certain that AgrD is a precursor peptide for AIPs and that AgrB functions as an enzyme in the post-processing of AgrD AgrB plays an important role in the synthesis of AIPs. AgrB plays an important role in the synthesis of AIPs. At least four types of self-inducible peptides have been identified and their structures are shown in the diagram below.

Translated with www.DeepL.com/Translator (free version)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3805138 short</partinfo>
+This part is an important part of the Agr system
-This part was made in order to transfer the S. aureus quorum sensing system into a BioBrick-compatible signalling mechanism.
+AgrD is a 138 bp sequence located in Staphylococcus aureus and has four different species, but all AgrD sequences include an Asp-Glu motif followed immediately by an AIP sequence, and the amino-terminal portion of AgrD has a conserved Ile-Gly motif. All precursor peptides of AgrD have an amphipathic α-helix fragment, which is responsible for anchoring AgrD to the cytoplasmic membrane.
+AgrB is a 567bp sequence that transcribes a transmembrane protein with multiple active centres, the topology of which is not well understood. H77 and C84 are essential amino acids for the synthesis of active AIP. It has been suggested that AgrB is a cysteine protease involved in the first step of the AgrD process and produces an acylenzymethioesterintermediate.
-In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.
+Although the information is not clear, it is certain that AgrD is a precursor peptide for AIPs and that AgrB functions as an enzyme in the post-processing of AgrD
+AgrB plays an important role in the synthesis of AIPs. AgrB plays an important role in the synthesis of AIPs. At least four types of self-inducible peptides have been identified and their structures are shown in the diagram below.
+Translated with www.DeepL.com/Translator (free version)