Difference between revisions of "Part:BBa K3757007"
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<partinfo>BBa_K3757007 short</partinfo> | <partinfo>BBa_K3757007 short</partinfo> | ||
− | Gene encoding the cyclotide precursor Oak1 (BBa_K3757001), C-terminally tagged with a c-myc tag ( | + | Gene encoding the cyclotide precursor Oak1 (BBa_K3757001), C-terminally tagged with a c-myc tag (BBa_K3757008). Regulated by the eukaryotic constitutive CaMV 35S promoter (BBa_K788000) and the 35S terminator polyadenylation signal (BBa_K1159307). In one Golden Gate cloning step, a sequence encoding a peptide can be cloned into the Oak1 precursor. This gene can then be co-expressed with CtAEP1 in Nicotiana benthamiana leading to expression of the peptide in cyclized form. |
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Revision as of 14:43, 28 September 2021
35S Oak1
Gene encoding the cyclotide precursor Oak1 (BBa_K3757001), C-terminally tagged with a c-myc tag (BBa_K3757008). Regulated by the eukaryotic constitutive CaMV 35S promoter (BBa_K788000) and the 35S terminator polyadenylation signal (BBa_K1159307). In one Golden Gate cloning step, a sequence encoding a peptide can be cloned into the Oak1 precursor. This gene can then be co-expressed with CtAEP1 in Nicotiana benthamiana leading to expression of the peptide in cyclized form.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1468
Illegal BamHI site found at 1858 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 66