Difference between revisions of "Part:BBa K3788021"

 
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<partinfo>BBa_K3788021 short</partinfo>
 
<partinfo>BBa_K3788021 short</partinfo>
  
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Promotor induced by SOS signal. This promotor is involved in the colA regulation system of E. coli. Colicine are toxins produce by E. coli, among her the Colicine A (ColA). Cai is a immunity protein against Colicine A (COlA). ColA is toxic by inserted into the Escherichia coli inner membrane, Cai will inhibited this processus. Cal coding by cal gene cal is a polypeptide needed for the release of colicin A in the ColA system.
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Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. In this contruction RFP permit to understand the mechanism, and Cal isn't able to have his lysis activity. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:12, 28 September 2021


Expression of GFP and of the timer device fused to the RFP protein

Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. In this contruction RFP permit to understand the mechanism, and Cal isn't able to have his lysis activity. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
    Illegal BamHI site found at 1521
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
    Illegal AgeI site found at 2849
    Illegal AgeI site found at 2961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 1351