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Tryptophanase could catalyze the reaction from a L-tryptophan to a indole and a pyruvate.
 
Tryptophanase could catalyze the reaction from a L-tryptophan to a indole and a pyruvate.
  
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===Usage and Biology===
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===Biological function===
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TnaA is a tryptophan clastic enzyme from E.coli substrain MG1655, it can decomposes tryptophan into many secondary metabolites like indole and pyruvate, and indole is the precursor of indigo.
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===Instruction===
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Tryptophanase is an endogenous enzyme of E. coli, and in most cases, it negatively affects the production of indigo derivatives (Tyrian Purple et al). Thus, in TnaA express process, TnaA deficient strain(TnaA(-)) is usually used as an expression chassis. We constructed the expression sequences in the TnaA(-) E. coli BL21 after codon optimization, and successfully transformed it into TnaA(-) E. coli BL21 to verify the protein expression.
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Revision as of 23:09, 21 October 2021


TnaA(tryptophanase from E.coli substrain MG1655)

Tryptophanase could catalyze the reaction from a L-tryptophan to a indole and a pyruvate.


Biological function

TnaA is a tryptophan clastic enzyme from E.coli substrain MG1655, it can decomposes tryptophan into many secondary metabolites like indole and pyruvate, and indole is the precursor of indigo.


Instruction

Tryptophanase is an endogenous enzyme of E. coli, and in most cases, it negatively affects the production of indigo derivatives (Tyrian Purple et al). Thus, in TnaA express process, TnaA deficient strain(TnaA(-)) is usually used as an expression chassis. We constructed the expression sequences in the TnaA(-) E. coli BL21 after codon optimization, and successfully transformed it into TnaA(-) E. coli BL21 to verify the protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]