Difference between revisions of "Part:BBa K3788011:Design"
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| + | The pelB and sfGFP sequences are made from respectively BBa_J32015 and BBa_I746916. And Aida-I sequence is taking from the UniProt data base with mutation on his coding sequence to get aspartic acid (Substitution D>N). | ||
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| + | After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25. | ||
Latest revision as of 21:45, 27 September 2021
The pelB and sfGFP sequences are made from respectively BBa_J32015 and BBa_I746916. And Aida-I sequence is taking from the UniProt data base with mutation on his coding sequence to get aspartic acid (Substitution D>N).
After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.