Difference between revisions of "Part:BBa K3866037"
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p>Τhis part consists of AndersonJ23115 promoter <bbpart>BBa_K3505012</bbpart>, N20-bglX <bbpart>BBa_K3866032</bbpart> and Double Terminator <bbpart>BBa_K3505017</bbpart>. By this way, as AndersonJ23115 is a strong constitutive promoter, bglx with the N20- signal peptide is constitutively expressed. The product protein is expected to be extracellular. | <p>Τhis part consists of AndersonJ23115 promoter <bbpart>BBa_K3505012</bbpart>, N20-bglX <bbpart>BBa_K3866032</bbpart> and Double Terminator <bbpart>BBa_K3505017</bbpart>. By this way, as AndersonJ23115 is a strong constitutive promoter, bglx with the N20- signal peptide is constitutively expressed. The product protein is expected to be extracellular. | ||
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| + | ===Design Notes=== | ||
| + | Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector <bbpart>BBa_K3505008</bbpart>and has overhangs compatible for GoldenBraid cloning. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 15:53, 27 September 2021
pAndersonJ23115:RBS-N20bglx-terminator
N20-bglX BBa_K3866032 under control of a constitutive promoter BBa_K3505012.
Usage and Biology
Τhis part consists of AndersonJ23115 promoter BBa_K3505012, N20-bglX BBa_K3866032 and Double Terminator BBa_K3505017. By this way, as AndersonJ23115 is a strong constitutive promoter, bglx with the N20- signal peptide is constitutively expressed. The product protein is expected to be extracellular.
Design Notes
Τhe coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha1R vector BBa_K3505008and has overhangs compatible for GoldenBraid cloning.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1700
Illegal AgeI site found at 1922
Illegal AgeI site found at 2111 - 1000COMPATIBLE WITH RFC[1000]